Ozone gas is an effective and practical antibacterial agent
American Journal of Infection Control
Volume 36, Issue 8,
October 2008, Pages 559–563
Research study by Manju Sharma, M.D. (Vioforce Systems, Inc.) and James B.
Hudson, Ph.D. (Dept. of Pathology and Laboratory Medicine, University of
British Colombia, Vancouver, B.C., Canada)
Bacterial infections continue to pose a threat to health in many
institutional and communal settings, and epidemics are frequent. Current
control measures are clearly inadequate; thus, there is a need for a simple,
effective, and safe way to decontaminate surfaces.
We evaluated the efficacy of a portable ozone-generating machine, equipped
with a catalytic converter and an accessory humidifier, to inactivate 15
different species of medically important bacteria.
An ozone dosage of 25 ppm for 20 minutes, with a short burst of humidity in
excess of 90% relative humidity, was able to inactivate more than 3 log10
colony-forming units of most of the bacteria, including Acinetobacter
baumannii, Clostridium difficile, and methicillin-resistant
Staphylococcus aureus, in both in a laboratory test system and simulated
field conditions. In many cases, complete eradication was achieved. Dried
and wet samples were equally vulnerable to the ozone. Inactivation of
bacterial samples dried onto soft surfaces (eg, fabric, cotton, filter
paper) were comparable with that observed for samples on plastic.
The ozone generator can provide a valuable decontamination tool for the
removal of bacteria in many institutional and communal settings, including
hospitals and other health care institutions.